LC/MSn high resolutionIon Max ESI Source & HCD Collision CellTested, with warranty.Spezifikationen:Routine FT mass accuracy60,000 resolution at 1 Hz scan cycleWide dynamic rangeParallel MS and MSn analysisMultiple dissociation techniques: CID, PQD and HCDMass Range m/z 50-2000, m/z 200-4000Resolution 60,000 at m/z 400 at a scan rate of 1 Hz,min. resolution 7,500 max. resolution >100,000 at m/z 400Mass Accuracy < 3 ppm external calibration, < 2 ppm internal calibrationSensitivity sub-femtomol range for peptidesDynamic Range > 4,000 within a single scanMS Scan Power MSn , for n = 1 through 10Price plus installation.

Like-new Orbitrap HRMS instrument, manufactured in 2021, rarely used.SpecificationsDynamic range:> 5,000:1 within a spectrumMass accuracy:< 3 ppm RMS – with internal calibrationMass range:40 to 6,000 m/zResolution:max. 480,000 at 195 m/zPolarity switching:One complete scan cycle in < 1 s(one complete scan each in positive and negative modes at a resolution of 60,000)Scan rate:max. 40 Hz at a resolution of 7,500 at 200 m/zSensitivity:MS/MS: 50 fg reserpine on column, S/N 100:1 SIM: 50 fg reserpine on column, S/N 150:1Refurbished, incl. fore-pump, data acquisition system, LED monitor(HPLC / UPLC optional upon request)

Device in stock and available for immediate delivery.Before we offer our devices for sale, they undergo a thorough cleaning and testing process. Each device is thoroughly inspected, cleaned, and tested to ensure that it is fully functional, so that you receive a product in perfect condition.The Thermo Fisher Q Exactive HF Mass Spectrometer, constructed in 2018 and in perfect condition, is available refurbished to manufacturer's specifications. Thoroughly tested, this instrument ensures exceptional performance and reliability. It integrates advanced Orbitrap mass analyzer technology, delivering unparalleled resolution and sensitivity for diverse proteomics applications. Ideal for laboratories seeking high-speed, accurate mass spectrometry, this instrument offers robust features for both identification and quantitation.Instrument Specifications:-    Scan speed up to 40 Hz with ultra-high-field Thermo Scientific™ Orbitrap™ mass analyzer technology-    Resolution of up to 240,000 (FWHM) at m/z 200-    High Capacity Transfer Tube (HCTT) and electrodynamic ion funnel for improved sensitivity-    Advanced Quadrupole Technology (AQT) for superior precursor isolation-    Advanced Active Beam Guide (AABG) for greater sensitivity and maximum robustness-    New Data Dependent Acquisition (DDA) strategy for bottom-up and top-down proteomics analysis-    Dynamic retention time adjustment in PRM for superior targeted quantitation-    Accelerated Higher-Energy Collisional Dissociation (aHCD) capabilities-    BioPharma option (paid upgrade) for enhanced analysis of intact proteins, protein-protein complexes, mAb subunits, and intact mAb. Includes Intact Protein Mode and High Mass Range (HMR) mode. 

Thermo Fisher Scientific Exploris 480 Orbitrap System- FAIMS Pro- Nano source- Fore-vacuum pump- Data system/PC with Xcalibur software3000 UHPLC System- 3500RS Nano ProFlow pump- WPS 3000TPL RS autosampler- SRD solvent degasser- Column oven with switching valveThe entire system dates from 2020 and is covered by a full service contract.Lightly used in the nanoflow / plasma proteomics application area.Original price: €1,140,000 (net)

Orbitrap Tribrid HRMS instrument, manufactured in 2014SpecificationsMass range:m/z 50–2,000, m/z 200–4,000, up to m/z 6,000 without precursor ionOTMS resolution:15,000–500,000 (FWHM) at m/z 200Scan rate:OTMSn up to 20 Hz, ITMSn up to 20 HzOTMS mass accuracy:< 3 ppm RMS with external calibration, < 1 ppm RMS with internal calibrationMS/MS electrospray ionization (ESI) ion trap sensitivity: 2 μL of a 50 fg/μL solution of reserpine(total 100 femtograms), injected at a flow rate of 500 μL/min, produces a minimum signal-to-noise ratio of 200:1 for the transition of the isolated protonated molecular ions at m/z 609 to the two largest products, m/z 397 and m/z 448, when the mass spectrometer is operated with unit resolution in full-scan MS/MS mode, m/z 165–615. An HESI III probe is required for the test.Dynamic range:> 5,000 within a single OTMS spectrumMS scan capability: MSn, for n = 1 to 10Synchronous precursor isolation, up to 20 precursors per MSn scanMultiplexing using the ion routing multipoleUp to 10 precursors per scan using the quadrupole mass filterPolarity switching:full cycle in < 1.1 s (one full scan in positive mode and one full scan in negative mode at a resolution setting of 30,000)ETD efficiency: ETD fragmentation efficiency of >15% (infusion of 1 pmol/μL angiotensin at 3 μL/min)refurbished, incl. forepump, control computer, LED monitor(HPLC / UPLC / nanoLC optional upon request)

Orbitrap Tribrid HRMS instrument, manufactured in 2017SpecificationsMass range:m/z 50–2,000, m/z 200–4,000, up to m/z 6,000 without precursor ionOTMS resolution:15,000–500,000 (FWHM) at m/z 200Scan rate:OTMSn up to 20 Hz, ITMSn up to 20 HzOTMS mass accuracy:< 3 ppm RMS with external calibration, < 1 ppm RMS with internal calibrationMS/MS electrospray ionization (ESI) ion trap sensitivity: 2 μL of a 50 fg/μL solution of reserpine(total 100 femtograms), injected at a flow rate of 500 μL/min, produces a minimum signal-to-noise ratio of 200:1 for the transition of the isolated protonated molecular ions at m/z 609 to the two largest products, m/z 397 and m/z 448, when the mass spectrometer is operated with unit resolution in full-scan MS/MS mode, m/z 165–615. An HESI III probe is required for the test.Dynamic range:> 5,000 within a single OTMS spectrumMS scan capability: MSn, for n = 1 to 10Synchronous precursor isolation, up to 20 precursors per MSn scanMultiplexing using the ion routing multipoleUp to 10 precursors per scan using the quadrupole mass filterPolarity switching:full cycle in < 1.1 s (one full scan in positive mode and one full scan in negative mode at a resolution setting of 30,000)ETD efficiency: ETD fragmentation efficiency of >15% (infusion of 1 pmol/μL angiotensin at 3 μL/min)refurbished, incl. forepump, control computer, LED monitor(HPLC / UPLC / nanoLC optional upon request)