• Reliable and simple to operate.• Universal detection of nonvolatile and many semi-volatile analytes.• Four orders of magnitude dynamic range (nonlinear).• Low ng lower limits of detection.• Typically < 2% R.S.D. response variability.• Response magnitude is minimally dependent on the analyte’s chemical properties.• Broad applicability.• Isocratic and gradient compatibility.

Temperature controlled enclosure for maintaining constant temperature and organizing coulometric array cells, pre-filters, column(s), manual injector valve, PEEK pulse dampers and biocompatible gradient mixers.Temperature range: + 5°C above ambient to 45°CTemperature accuracy: < 0.5°C

MODELCOULOCHEM II HPLC SYSTEM DETECTOR (SCATTERED LIGHT)  MODELUV/VIS MODEL 522 Wavelengths, nm (OPERATING)190-800programmable Drift, absorb/hr (DETECTOR)<,0.0002 Noise, aufs (DETECTOR)<,±0.00001 Flow cell vol, µl (DETECTOR)0.25-15 Cell pathlength, mm (DETECTOR)2-10, varies with Avail absorb, aufs (DETECTOR)0.0005-3 Recorder output (DETECTOR)Selectable Event marker (DETECTOR)Yes SYSTEM CONFIGURATION  Design (SYSTEM CONFIGURATION)Modular, isocratic std configurationDepends on config SAMPLE INJECTORAutosampler - for details please see comments.542 Autosampler Fixed loop or  variable (Fixed loop or)Variable Manual or automatic (SYSTEM CONFIGURATION)Automatic SAMPLE VOLUME, µL1 Amount of vials (SYSTEM CONFIGURATION)24,84,96 Injections/sample (SYSTEM CONFIGURATION)9 Perfomance time, min (SYSTEM CONFIGURATION)9hr 59 min 59 sec Maximal pressure (SYSTEM CONFIGURATION)5000 Auto flushing (SYSTEM CONFIGURATION)Yes Standard vial (SYSTEM CONFIGURATION)4types Kind of vial (SYSTEM CONFIGURATION)0.5,1.5, 4,10mL DATA MANAGEMENT  MODELELITE EZCHROM FOR Channels (POWER NEEDED)4 DISPLAYMS Windows-based Chromatograms,  no. stored (Chromatograms,)Unlimited Optional storage (DATA MANAGEMENT)Yes PUMPESA-582 Type (CONFIGURATION)Dual piston, Pump heads (PUMP)2 Flow range, ml/min (ELUENT PUMP)Up to 10 - please see comments for detailed specifications.0.001-9.999 Flow increm, ml/min (PUMP)0.001 Flow precision, % (ELUENT PUMP)0.3 Flow accuracy, % (ELUENT PUMP)1 Max pressure, psi (PUMP)5000 Max/min pressure  switches (Max/min pressure)Yes Gradient capability (PUMP)Optional with second Solvents (PUMP)1isocratic OTHER DETECTORS (DATA MANAGEMENT)Coulochem Electro- COLUMN (SPECIFICATIONS)  MODELMETA-250 : MICRODI- Construction (ROTOR)Stainless steel Length/internal  Diameter, mm (UMBILICAL CORD)250:150:80:150 Particle size, µm (SPECIFICATIONS)37320 Particle type (COLUMN)Silica, polymeric Sorbent type (SPECIFICATIONS)  Reverse phase (COLUMN)C18, C8, proprietary OTHER SPECIFICATIONS (AIR SUPPLY)Manual injector. CE MARK (MDD) (Interference compensation)Yes

MODELCOULOCHEM II HPLC SYSTEM DETECTOR (SCATTERED LIGHT)  MODELUV/VIS MODEL 522 Wavelengths, nm (OPERATING)190-800programmable Drift, absorb/hr (DETECTOR)<0.0002 Noise, aufs (DETECTOR)<±0.00001 Flow cell vol, µl (DETECTOR)0.25-15 Cell pathlength, mm (DETECTOR)2-10, varies with Avail absorb, aufs (DETECTOR)0.0005-3 Recorder output (DETECTOR)Selectable Event marker (DETECTOR)Yes SYSTEM CONFIGURATION  Design (SYSTEM CONFIGURATION)Modular, isocratic std configurationDepends on config SAMPLE INJECTORAutosampler - for details please see comments.542 Autosampler Fixed loop or  variable (Fixed loop or)Variable Manual or automatic (SYSTEM CONFIGURATION)Automatic SAMPLE VOLUME, µL1 Amount of vials (SYSTEM CONFIGURATION)24,84,96 Injections/sample (SYSTEM CONFIGURATION)9 Perfomance time, min (SYSTEM CONFIGURATION)9hr 59 min 59 sec Maximal pressure (SYSTEM CONFIGURATION)5000 Auto flushing (SYSTEM CONFIGURATION)Yes Standard vial (SYSTEM CONFIGURATION)4types Kind of vial (SYSTEM CONFIGURATION)0.5,1.5, 4,10mL DATA MANAGEMENT  MODELELITE EZCHROM FOR Channels (POWER NEEDED)4 DISPLAYMS Windows-based Chromatograms,  no. stored (Chromatograms,)Unlimited Optional storage (DATA MANAGEMENT)Yes PUMPESA-582 Type (CONFIGURATION)Dual piston, Pump heads (PUMP)2 Flow range, ml/min (ELUENT PUMP)Up to 10 - please see comments for detailed specifications.0.001-9.999 Flow increm, ml/min (PUMP)0.001 Flow precision, % (ELUENT PUMP)0.3 Flow accuracy, % (ELUENT PUMP)1 Max pressure, psi (PUMP)5000 Max/min pressure  switches (Max/min pressure)Yes Gradient capability (PUMP)Optional with second Solvents (PUMP)1isocratic OTHER DETECTORS (DATA MANAGEMENT)Coulochem Electro- COLUMN (SPECIFICATIONS)  MODELMETA-250 : MICRODI- Construction (ROTOR)Stainless steel Length/internal  Diameter, mm (UMBILICAL CORD)250:150:80:150 Particle size, µm (SPECIFICATIONS)37320 Particle type (COLUMN)Silica, polymeric Sorbent type (SPECIFICATIONS)  Reverse phase (COLUMN)C18, C8, proprietary OTHER SPECIFICATIONS (AIR SUPPLY)Manual injector. CE MARK (MDD) (Interference compensation)Yes

MODELCOULOCHEM II HPLC SYSTEM DETECTOR (SCATTERED LIGHT)  MODELUV/VIS MODEL 522 Wavelengths, nm (OPERATING)190-800programmable Drift, absorb/hr (DETECTOR)<0.0002 Noise, aufs (DETECTOR)<±0.00001 Flow cell vol, µl (DETECTOR)0.25-15 Cell pathlength, mm (DETECTOR)2-10, varies with Avail absorb, aufs (DETECTOR)0.0005-3 Recorder output (DETECTOR)Selectable Event marker (DETECTOR)Yes SYSTEM CONFIGURATION  Design (SYSTEM CONFIGURATION)Modular, isocratic std configurationDepends on config SAMPLE INJECTORAutosampler - for details please see comments.542 Autosampler Fixed loop or  variable (Fixed loop or)Variable Manual or automatic (SYSTEM CONFIGURATION)Automatic SAMPLE VOLUME, µL1 Amount of vials (SYSTEM CONFIGURATION)24,84,96 Injections/sample (SYSTEM CONFIGURATION)9 Perfomance time, min (SYSTEM CONFIGURATION)9hr 59 min 59 sec Maximal pressure (SYSTEM CONFIGURATION)5000 Auto flushing (SYSTEM CONFIGURATION)Yes Standard vial (SYSTEM CONFIGURATION)4types Kind of vial (SYSTEM CONFIGURATION)0.5,1.5, 4,10mL DATA MANAGEMENT  MODELELITE EZCHROM FOR Channels (POWER NEEDED)4 DISPLAYMS Windows-based Chromatograms,  no. stored (Chromatograms,)Unlimited Optional storage (DATA MANAGEMENT)Yes PUMPESA-582 Type (CONFIGURATION)Dual piston, Pump heads (PUMP)2 Flow range, ml/min (ELUENT PUMP)Up to 10 - please see comments for detailed specifications.0.001-9.999 Flow increm, ml/min (PUMP)0.001 Flow precision, % (ELUENT PUMP)0.3 Flow accuracy, % (ELUENT PUMP)1 Max pressure, psi (PUMP)5000 Max/min pressure  switches (Max/min pressure)Yes Gradient capability (PUMP)Optional with second Solvents (PUMP)1isocratic OTHER DETECTORS (DATA MANAGEMENT)Coulochem Electro- COLUMN (SPECIFICATIONS)  MODELMETA-250 : MICRODI- Construction (ROTOR)Stainless steel Length/internal  Diameter, mm (UMBILICAL CORD)250:150:80:150 Particle size, µm (SPECIFICATIONS)37320 Particle type (COLUMN)Silica, polymeric Sorbent type (SPECIFICATIONS)  Reverse phase (COLUMN)C18, C8, proprietary OTHER SPECIFICATIONS (AIR SUPPLY)Manual injector. CE MARK (MDD) (Interference compensation)Yes

MODELCOULOCHEM II HPLC SYSTEM DETECTOR (SCATTERED LIGHT)  MODELUV/VIS MODEL 522 Wavelengths, nm (OPERATING)190-800programmable Drift, absorb/hr (DETECTOR)<0.0002 Noise, aufs (DETECTOR)<±0.00001 Flow cell vol, µl (DETECTOR)0.25-15 Cell pathlength, mm (DETECTOR)2-10, varies with Avail absorb, aufs (DETECTOR)0.0005-3 Recorder output (DETECTOR)Selectable Event marker (DETECTOR)Yes SYSTEM CONFIGURATION  Design (SYSTEM CONFIGURATION)Modular, isocratic std configurationDepends on config SAMPLE INJECTORAutosampler - for details please see comments.542 Autosampler Fixed loop or  variable (Fixed loop or)Variable Manual or automatic (SYSTEM CONFIGURATION)Automatic SAMPLE VOLUME, µL1 Amount of vials (SYSTEM CONFIGURATION)24,84,96 Injections/sample (SYSTEM CONFIGURATION)9 Perfomance time, min (SYSTEM CONFIGURATION)9hr 59 min 59 sec Maximal pressure (SYSTEM CONFIGURATION)5000 Auto flushing (SYSTEM CONFIGURATION)Yes Standard vial (SYSTEM CONFIGURATION)4types Kind of vial (SYSTEM CONFIGURATION)0.5,1.5, 4,10mL DATA MANAGEMENT  MODELELITE EZCHROM FOR Channels (POWER NEEDED)4 DISPLAYMS Windows-based Chromatograms,  no. stored (Chromatograms,)Unlimited Optional storage (DATA MANAGEMENT)Yes PUMPESA-582 Type (CONFIGURATION)Dual piston, Pump heads (PUMP)2 Flow range, ml/min (ELUENT PUMP)Up to 10 - please see comments for detailed specifications.0.001-9.999 Flow increm, ml/min (PUMP)0.001 Flow precision, % (ELUENT PUMP)0.3 Flow accuracy, % (ELUENT PUMP)1 Max pressure, psi (PUMP)5000 Max/min pressure  switches (Max/min pressure)Yes Gradient capability (PUMP)Optional with second Solvents (PUMP)1isocratic OTHER DETECTORS (DATA MANAGEMENT)Coulochem Electro- COLUMN (SPECIFICATIONS)  MODELMETA-250 : MICRODI- Construction (ROTOR)Stainless steel Length/internal  Diameter, mm (UMBILICAL CORD)250:150:80:150 Particle size, µm (SPECIFICATIONS)37320 Particle type (COLUMN)Silica, polymeric Sorbent type (SPECIFICATIONS)  Reverse phase (COLUMN)C18, C8, proprietary OTHER SPECIFICATIONS (AIR SUPPLY)Manual injector. CE MARK (MDD) (Interference compensation)Yes

Wavelength range200 to 890 nm (excitation) 210 to 900 nm (emission) Wavelength accuracy±3 nm Bandwidth20 nm Wavelength reproducibility±0.25 nm SensitivitySignal to noise ratio > 1000 (Raman spectrum of H2O) Measuring range0.001 to 10,000 EU Data acquisitionUp to 80 Hz Flow cell designAxially illuminated Cell volume< 2 μL Pressure limit500 psi Materials with liquid contactFused silica, FEP, PEEK Dimensions and weightWidth: 13.5 in (34.3 cm)Height: 8.5 inches (21.6 cm)Depth: 50.8 cm (20.0 in.)

Wavelength range200 to 890 nm (excitation) 210 to 900 nm (emission) Wavelength accuracy±3 nm Bandwidth20 nm Wavelength reproducibility±0.25 nm SensitivitySignal to noise ratio > 1000 (Raman spectrum of H2O) Measuring range0.001 to 10,000 EU Data acquisitionUp to 80 Hz Flow cell designAxially illuminated Cell volume< 2 μL Pressure limit500 psi Materials with liquid contactFused silica, FEP, PEEK Dimensions and weightWidth: 13.5 in (34.3 cm)Height: 8.5 inches (21.6 cm)Depth: 50.8 cm (20.0 in.)

Two high resolution quadrupole analyzers (MS1/MS2), plus prefilters to maximize resolution and transmission while preventing contamination of the main analyzers.T-Wave™1 enabled for optimal MS/MS performance at high data acquisition rates.Low noise, off axis, long life photomultiplier detectorDigital dynamic range up to 4 x 10^6.Full scan MSProduct ion scanPrecursor ion scanConstant neutral lossSelected Ion Recording (SIR)Multiple Reaction Monitoring (MRM)Survey scan modes: Full scan MS triggered product ion scanMass range 2 to 2048 m/zScan speed Up to 10,000 Da/sExamples of achievable acquisition rates 10 scans per second (m/z 100 to 1000), 20 scans per second (m/z 50 to 500)Mass stability Mass drift is <0.1 Da over a 24 hour period 

RI Range1.00 to 1.75 RIU Typical flow rate0.2 ~ 3.0 ml/min Noise≤ 5 x 10¹⁰ RIU Auto zero range40 x 10⁵ RIU Auto zero resolutionto 5 x 10¹⁰ RIU Recorder output± 1 V Linear dynamic range80 x 10⁵ RIU Drift rate4 x 10⁸ RIU Flow rate range0.2 ~ 3.0 mL/min Cell volume9 μl Cell pressure6 kg/cm² (84 psi) Volume into cell24 μl Temperature control35°C ~ 55°C Digital interfaceRS-232C Digital inputPurge, Auto Zero Dimension297 × 198 × 495 mm (WxHxD) Power requirement110/220 ± 10% VAC. 50-60 Hz. Weight15 kg